Interesting article on ANDi there... When I talked to Anthony years ago he told me ANDi did fluoresce... wonder if that was before or after that article was out...
I did not say you HAD to have it I just said it would make the process easier if you were looking to insert it at a specific loci. Like if you wanted just the alien heads to fluoresce...You do NOT have to know the entire genome of an animal in order to introduce GFP genes.
I am sorry, I hate to sound arrogant but I hold a PhD in molecular genetics.Genetic engineering is much messier than you realize.I realize full well how messy genetic engineering can be (and for the most part is.) However, I also realize that, with the advent of modern and next-gen sequencing and refined techniques, it need not be as messy. Synthetic biology is not that that far away and not nearly as messy.
I think it might take a bit more than that. I could well be mistaken but I think you would need to hit the egg much earlier on, while it was still in the mother. But I could be mistaken on that. I have not seen any work on TG animals that retain their eggs for a period after fertilization, that may complicate the matter some.And all you really need is a freshly laid ball python egg, and a male and female ball python, to get started. (And a lot of money and special equipment).