Here is what happened to me. I was handling my snake 10-20 minutes a day about a month ago, then I started handling more (upto 25 mins). Then I started doing it twice, once at 5pm, then again around midnight. Soon I noticed my bp got less restless and started hissing a lot more and once even snapped into the striking S and struck at me few times. So I eased down, and since then have only been handling him max 20 mins per day. I've also given him days off several times and he's back to his normal super calm, super cool ways

The point of the story is that once you're handling too much your bp will let you know by his behavior.