Never leave substrate in once you determine mites. You must clear everything out and either sanitize it or toss it. Treat the tubs and treat the snakes for no less than a month. The best thing is to have paper towels to easily change substrate and monitor for new mites.
The mite lifecycle needs to be killed which means at least a month of treating and cleaning on a consistent basis. Provent a mite alone is not sufficient, get nature's chemistry reptile spray as well to treat the actual snake. That one-two punch is the best way.