Some people say no, but my experience says yes - and that if the can smell a female they'll absolutely fight. If one is removed and put with a female, it can't be put back with the others. I've heard on here that the same is true for rats - I've never had that happen with rats. I was going to separate my male rats, just in case, but they don't do well by themselves so I put them back together and they were delighted to see each other again. Male mice, however, just from personal experience, will kill each other even if they're raised together, if any of them are taken out and put back in, or if there are female mice around, particularly if they are in heat.