i have thawed, refreezed, and thawed again. i've had animals eaten these thawed out (twice) feeders with no ill affects. however the second go around there may be some slight issues......
when you refreeze and thaw the tissue weakens even more....which usually results to exploding stomachs in mice. since rats have a bit more thicker skin it doesn't happen that often, but the possibility is always there.
also on the second go around of thawing there's always that slight stench of "death smell" which some people might not be able to handle.
just remember if you notice any bloating....toss the feeder. once the bloating starts it will continue until the gas i released some how....either by the mouth, anal cavity, or by rupture.
bad smell is doable.....bloating is not.
Pitoon