As others have said, regular brief handling sessions should get him back to being more comfortable with it.
If you are afraid of being bitten, gloves go a long way, but so does just letting him bite you once and realizing it isn't that bad. If my husband or I get struck at, the thing we worry about is if our instinctive pulling away from something that hurts or is scary will kick in before our knowledge that it neither hurts nor is scary does, because pulling away could hurt the snake's teeth. That sentence was awkward but hopefully you'll understand what I meant.
A trick I learned from someone who works mostly with colubrids is that if a snake looks like it is thinking about striking, move your hand towards it, presenting it with the wide, flat back of your hand. Usually they'll decide not to strike, but if they do they don't get a hold so it eliminates the possibility of either of you getting hurt.