Now that's a tough one, but actually once you get them all trained, even removing them from the cage shouldn't make them forget to go in the litter box.
Baby rats are very difficult to litter train. I suppose it is possible to train them, but they will always have accidents. Which might I add might make your adults slip up.
I would completely scrub out your entire cage (have to remove that bathroom smell), remove all of the youngsters (place them in a temporary enclosure and return the adults back into the cage with the litter and litter box. Take a couple of days to train the adults, getting good litter that they naturally like to poo on is the key to success. Then, return the youngsters gradually back to the cage, training as you go.
Remember, when you are training them to place all of their poo in the litter box. This will give them a subtle hint.
Keeping the cage spotless during training is mandatory! Making sure there are as little bathroom smells as possible.
I've never trained such a large group of younger rats or ones that were removed from their cage, but I hope that helps.![]()