Greetings,
If the perlite is 'clean' and has been dry and not exposed to moisture then it should be fine. Note: The egg boxes should be disinfected prior to use (good: rubbing alcohol, better: diluted bleach solution).
If you are using a standard egg box setup with perlite above a layer of water then just make sure the perlite is deep enough that you can rest the eggs on the top so they remain humid, but, do not touch the water below. I recommend using distilled water.
Make sure the incubator is up to temperature before adding the eggs. Maternal incubation is fine in the very early stages, but, gets more problematic as the embryos grow.
Living systems can often be unpredictable. Make sure to have fun!![]()