Thank you for your reply. So far, the 3 female mice are getting along very well with all three litters and everything is going well. The wheel is no problem, neither for the mice nor the ASF, as the pups are taken well care of (except the fact that one rat tries to steal the pups of the other rat).

Would you recommend that I separate the first female rat with her 13 older pups into another setup until the pups are fully weaned and then feed off the female rat (the "mom")?

As the other two female rats get well along and even nurse the first litter I do not think it would be a good idea to take out the second female rat with her newborn babies as it might not be able to introduce her back to the colonie afterwards?