Oh they absolutely have different personalities. My two girls are like night and day. Ana Gwyn (my redhead pastel) is a total sweetheart, you can hold her all day and she's perfectly happy. She is also ALWAYS under a hide but refuses to use anything made for snakes - only plastic dog dishes for her, thanks. Then there's Tonks, my chocolate enchi, who WILL NOT USE A HIDE AT ALL. Ever. Neeeever. She has some, but will push them out of the way to sit on the UTH, or like now she's squinched up between the hide and the tank glass. I've tried tricking her into hides by putting one on her while she was sleeping, she will just wake up and glare at me then move. She recently spent two whole days just curled up beside her water dish, not on the UTH or anything, just hanging out by the pool I guess. Tonks is also a lot more nervous about being held, unless you're my niece, who can hold her for hours and it's all cool. They're very different about food, too - Ana Gwyn just gently takes her rat and barely even wraps it, if at all, while Tonks goes WILD as soon as she smells her mouse and strikes like crazy until she gets lucky.
I'm getting a baby male bullsnake (one of Snake Discovery's false ghost clutch, I'm excited!) in a few weeks, and I'm very much looking forward to learning a third snake's personality!