I defrost mine in warm water from the tap, treated with ReptiSafe. Used to keep them in an airtight ziplock bag while they were in the water, but now I just leave them in the warm water to defrost without the bag. This way, I can get some water soaked into the rat's body while it thaws out. At least, thats the idea. Then I just take it out, dry it a bit with paper towel, and feed.