If mites are present best no to use substrate like aspen, mulch, etc. Use paper towel or unprinted newspaper. Remove water bowl, spray enclosure, decor, hides lightly and wait a hour before adding animal and water. I like to wait 2 hours personally. When you spray you don’t want things to be saturated. I’ve found Pam usually clears things in a week at most. Hope this helps
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The only reason why I sprayed the substrate was to prevent future infestations- he recently got out of his mite problem. I just checked the substrate and it's almost completely dry. But it wouldn't be safe to use?