It won't be the exact same, but should give you a more accurate reading.
What I would do is put some paper towels at the very bottom, then put the substrate on top. Wait an hour or two then get to the bottom where the paper towels are and use the gun to shoot the paper towels. I'd put 3-4 down though so you get an accurate reading. Then you can shoot the bottom and get an idea of the difference.
When I use heat tape on the pvc, I have to keep the probe set to 80, because under all the substrate, that bottom of the pvc enclosure was around 88. Before I put the substrate down, it was reading around 82-83, so with the substrate it raised it around 5 degrees. Now I don't know if the uth heater will do that, but I just wanted to give you an idea.









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