I use tubs for quarantine, I spray the empty tub (bottom, sides, lid) then let dry for 24 hours (usually 48 hours before I introduce the animal just to be safe but as long as it's dry and doesn't smell you should be fine to put the animal back in). I use paper towel for substrate, a hide and water bowl. That's it. I feed in the cage, haven't had any issues. I usually give new animals a week to settle in before attempting to feed. I only treat the one time and have brought animals into QT with mites and this eradicated them. I've heard it was fine to spray the substrate, I simply just do the tub and it's worked for me. Good luck! mites are such a pain.