Just make sure you are fully thawing the stuff. Smaller rats isn't that big of deal as you can easily tell if they are fully thawed but for big stuff, you want to make sure the insides are thawed and not frozen still. That's why I use warm water. Easier to tell if 2XL guinea pigs and large rats are fully thawed as I just kind of squeeze them a little and you can easily tell as you will feel a big temp difference.