Only two days to settle in isn't much, most people give a solid 5 to 7 days with no interactions besides spot cleaning and water changes before attempting feeding or handling. Your husbandry sounds on point so it may possibly be stress from not allowing her time to acclimate.
Could also be that the mouse isn't hot enough. It needs to be nice and warm to the touch, almost hot, when you offer it... and from what it sounds like leaving the mouse in warm water for 45 minutes probably didn't do the job, especially since it will lose a lot of the heat between you taking it out of the water walking over and offering it to the snake. 98 to 101 degrees is average body temp for a rat, and I usually achieve good temps by holding the rat under the tap with the water running hot (but not uncomfortably so) for a minute or two.