You will need to take everything out of the enclosure. Either throw away the substrate or bake it in the oven at 150*F for half an hour to kill the mites and eggs hiding in it. Don't use natural substrate again until you've eradicated the infestation, which takes about two months.
When treating for mites your snake should have:
- Paper substrate in the enclosure
- Two hides, one on the warm side and one on the cool side
- One water bowl.
Treat the paper, enclosure, and hides with the PAM and let everything dry before allowing your snake to come into contact with it. DO NOT TREAT THE WATER BOWL. Keep a supply of treated and dried paper and hides handy so you can swap them quickly when your snake makes a mess and the enclosure needs cleaning.
If you have more than one snake treat everything in your collection.