My first ball python

[Regius_049]

1. I would like to know what types of substrate I should be using? I'm currently using a loose coconut fiber substrate with a carpet mat underneath. I am also running a reptile tank heater underneath (on one side of course) during the night when my lamp is off. Should I be concerned about burning the belly of my snake?

You should be using a thermostat with an under tank heater to monitor and control the temperature. 88-92 degrees is typically considered "ideal". For new ball pythons, I universally recommend paper towels. They are easy to change out and spot potential problems with a new animal.

2. Also, should I use a heat lamp on one side of the tank on a 12/12 cycle? I've heard yes and no. If yes, what watt should I use and how do I keep my snake from accidental burns?

You can put your snake one a day/night cycle. It is not required, but it won't hurt anything. I would use whatever wattage gives you an ambient around 76-80 degrees. Put the bulb outside the cage with screen or wire separating it from the snake. Alternatively, there are "in the enclosure" wire cagings that can be used with heat bulbs.

3. Should I spritz my tank daily in order to keep the humidity at a comfortable level? I have a humidity gauge coming in the mail.

I generally do not recommend spraying/misting enclosures designed to hold humidity unless you change paper towels regularly or allowed the enclosure to dry out entirely. Leaving constantly damp substrate makes for ideal conditions for mold and bacterial growth. I typically do not like to mist enclosures unless they dry out easily or are bio-active. I recommend increasing water bowl size or placing it under heat if you need more humidity.

4. When I feed my snake, what is the best way to keep him isolated so that I do not disturb him? I want him to have a comfortable set up so that he can digest his meal.

No need to overthink this. Once he eats just leave him be in his enclosure for 24-48 hours. Provided he has adequate temperatures, he will digest his meal just fine.