Only if they aren't pregnant again, which they might be depending on how late exactly you separated them. Note most of my knowledge is from doing research, my colony is still very new and I haven't needed to do any separations yet (other than old litters of course).

But I have heard it is possible, though it might be a good idea to wait a week or two after they are done weaning before trying to reintroduce them, if you are sure they aren't ready to pop again.