The appropriate concentration is 1: 1000 . That is a 3cc dose of f10sc to a 1000cc of distilled water. Remember this is in no way a substitute for actual herp vet examination and treatment. It's usually for a mild case of RI but is better facilitated as a adjunct to other vet supported therapy. The ventilation holes should be placed in a way that there is adequate oxygen exchange for the animal. Make sure to place paper towels in the tub the reptile is in so as to absorb the condensation and droplets from the nebulization. Then place the tub in a larger tub with the connections of the tubing for the procedure. Make sure to advise your vet about the procedure and get his approval beforehand.