Just do it quick and be forceful about it. Some twitching might happen even though its already dead. Gloves would be alright to use if you're worried about it biting you. If the rat is calm and you do it quick enough though then it won't get the chance to bite you. In the video I watched, the guy had something for the mouse to hold onto with its front paws so that gave it a pencil to hold onto. It works alright and it was quick.

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