32 quarts is very big how many rats do you want to euthanize at once?
If you do need such a large kill tank, you do not want a paintball canister it will be too small, you will have to refill it all the time, not even sure it will do the job.
Smaller kill chamber are much more efficient.
In a small tub (7 quarts) I euthanize 2/3 breeder rats 5/7 medium and about 10/14 small at the time.
If you have such a large tub but hardly any rats it will take you longer and you will need much more CO2.
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