Yeah, not sure about with mice, but i just had to show a breeder holdback rat how the valves work. When you put the mice in the rack, make sure there is a drop of water around the tip of the valve. Just push it with your finger. It may take a few tries, but they should eventually catch on, and at least with rats when one gets it the others will learn. Just make sure you some moisture on the tip of the valve, thats what happened with mine. Cage was empty for a bit, valve was dry, and he was taken from a holding bin with a bottle. Once i got a drop hanging from the valve and showed him where it was he picked it right up.