Hi,
We normally take the count from the pre-lay shed that happens after ovulation.
There is still some variation but mine seem to go around 32-35 days after that shed.
I use a plastic grid on top of the incubation medium ( the so called substrateless method ) rather than bedding them down in it but you shouldn't run into any problems as long as your incubator has been up and running so the temps inside are stable and tested.
Hope this helps.![]()