I don't think this will affect your relationship with your snake/her trust towards you, she probably didn't even connect that it was you who was causing the spray. I hope this isn't offensive to anyone, but I'm not even sure if snakes can tell humans apart very well if at all (PLEASE tell me if I'm wrong I'm not trying to spread false info)... it's more like snakes can tell if a person is confidently, comfortably handling them vs. handling them in a way that scares or hurts them