The older one may have been too stressed, or otherwise unable to take care of herself and all those babies. The younger could have had a uterine infection brewing. Her death/symptoms (blood around the urogenital area) could have just been coincidental with the introduction of the male.
As for running a necropsy... unless you plan on paying for biopsies and lab tests, just do the gross necropsy yourself. It isn't too hard, especially if you have ANY experience with dissection at all. If you need help knowing what you are looking at, I can send you links to sites with good information, or you can take pictures and send me and I'll take a look to see if I can spot anything. You may not be able to determine cause of death, but you can rule out some causes. It's a heck of a lot cheaper too. (A vet I used to work with charged $70 for a gross necropsy, and basically told me the same thing I could have determined by myself for free.)
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