If it were me I would remove all remaining snakes off the aspen and sterilize their bins. Put them on newspaper or paper towel and monitor them all closely.
Sounds to me like it was related to the aspen. Even if it had been cedar or pine, it wouldn't have killed the 5 snakes, but would have made them sick over a much longer period of time. They likely wouldn't have upped and died - but would have displayed symptoms of respiratory distress. I'm thinking perhaps some pesticide or other chemical was leaked into the wood shavings, perhaps in transport to the tractor supply, perhaps at the tractor Supply, who knows.
But the aspen seems to be the link, to me. If that's the only thing you have changed recently about their environment.
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