I had one boa with mites and he was in a completely different room, in a 28 qt tub with no substrate lol nothing in my collection got them it was only him all by himself. Its pretty hard for error. remove animals and water bleach, rinse, dry, spray, dry/air out. replace animal repeat in 2 weeks. by 2 weeks they would show up again Im assuming it was eggs that had hatched on the animal?? anyway did this for 2 months with him, gave up and did nix not a problem since. I don't know?? most people do recommend pam and use it with great success, but I know quite a few that prefer the nix over pam. I am now one of them