When my collection got mites, my spider and my burm both soaked incessantly, and as they said above it does generally take a week or two of treatment to really be sure youre rid of them. While I dont think 24 hours without water dehydrate him to any terrible degree, he might just still have been itchy, and at that point any degree of dryness would agitate it even more. I wouldnt worry about it unless he's still soaking in 2-3 weeks.