Well the $165 is the fee for the lab tests and diagnosis she charged $45 to stick the swab down the trachea. I did mention a lung wash and she said the swab method puts allot less stress and is more safe for the snake. Given the effects of the anesthetics and the decreased capacity of the lungs from the RI i guess it could cause the snake to stop breathing. As for the lesser not having enough saliva it was really up to me but she said the results would not be as effective and leaded me away from it.