Yeah. You have to separate them by sex otherwise, they might start to breed.
I haven't seen any of my rat pups breed as young as 5 weeks, but better safe than sorry.
They get weaned by 4(ish) weeks. So smaller than small. I don't know how many grams, but this is what my weans look like:
The little one on the right is ~3 weeks old.
The middle is weaned.
The big one is ~ 5 months old.
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