So I'm guessing that since your working with one rat you don't want to go through the expense of buying a co2 tank or finding dry ice. You can use common household products to create co2. There are plenty of guides online using Baking soda and vinegar. It works well enough to euthanize a rat. I'm not sure if it would produce enough Co2 over a sustained period of time to kill a larger grouping but it might. Please don't put them in a bag and bash them around because that's just plain animal abuse. Sorry if you disagree but people keep rats as pets and to them doing that is akin to placing a puppy in a bag and slamming it all over the place.