Do ball pythons have taste buds?
If so, what are they used for?
After you've done the research and answered these two questions, ask yourself this:
Does fresh really matter to a ball python? If so, as opposed to what?
Then, will a ball python be able to discern the difference - with it's "palate" - between a live prey item and a f/t one sufficiently heated up?
Once you've done that research, we will then have something interesting to discuss. Until then, we don't.