I let the tubs sit for at least 30 minutes just to be sure. It dries pretty fast so you don't have to wait forever. When I did it last it was in the summer so I put the tubs outside under the tree on a 90 degree day. Putting the snake in a tub that has been Pamed should take care of any mites that may be on the snake. If you ever get anything really bad I would also get Reptile Relief to spray directly on the snake to take care of the problem a bit faster.