They open their eyes at 2 weeks old and I wean rats at 3 1/2 - 4 weeks old, typically they are about 35 grams at this age.
If I'm holding back rats to raise to become breeders I cull the litter down to 4-5 kits and by the time those are weaned they are HUGE, lol.
I like my holdbacks to stay with momma rat till they are at least 4 weeks old or even a bit older if possible.