I've never heard of a "leopard" gene...can you link to some info about it?
As for the pied gene...I've yet to see definitive proof that the "markers" are absolute. There may be some genetic visual trace for het pieds...but those same visuals CAN appear in normals as well....and I have seen het pieds that don't show any of the classic "markers".
That said...the breeders I've spoken to who regularly breed pieds seem pretty confident in their ability to spot the hets in a pos-het clutch...but they still sell them as poss-hets. It's simply not as clear-cut as the het ivory, for instance, or het red axanthic.