Herpmanout82,
I am new at breeding mice but it seems to me that they open eyes at around two weeks old. I remove them to a spare tub at about 3 to 4 weeks (one tub for males and one for females) depending on if they are eating and drinking from the water bottle. If the male was in with the female when the litter was born, then it is likely that she is pregnant again. She may have more about 21 days from when the first litter was born. They are very prolific once you get them going.