They will need longer than that apart, as the females will likely only have stopped laying just prior to March or April (or may still be laying depending on how long they retain sperm) if separated in Setember or October. If you cool them temperature wise they may stop laying, but can still retain sperm and start laying again in the spring when it warms up, so keep that in mind too. A proper rest is one where they have stopped laying completely and then had at least 6 months or more to rest.