Definately make sure the temp is stabilized. Also watch the temp close-spikes up or down aren't good. If you have a big clutch your temps may rise as the eggs develop. Some may argue,but if your incubator is set up properly with a thermostat/temp controller,and up to the correct temp, I don't see a reason to wait to put the eggs in it. The overnight wait wouldn't be a bad idea just to make sure the incubator is functioning properly. Congrats on the clutch! Jason Bowden