Well, I'm highly allergic to most beddings. Beddings that I'm not allergic to give me problems due to dusts, and the ones that I CAN live with are expensive for a small time "operation". So, I'm starting my colony without any type of bedding. I will of course give paper towels for the females with litters/about to drop litters, but this will be the extent of it. Has anyone else tried this or anything similar? So far (about two weeks into it) it seems to be going ok. I simply move the mice to another tub, then hose out the old one. The one thing I can see having problems with is the litter tubs. I can see possible problems with cleaning out the tubs when the litters are young, especially considering that I plan on housing two females per tub, which will probably drop at different times, causing different aged babies, older ones will produce more waste, while younger ones will prevent me from cleaning the cage. So, I will probably have to figure something else out for the litter tubs. I had 1 male to 3 females in a 20qt sterilite tub and had to spray it out approximately every 2-3 days, but I haven't had the larger colony in the larger tub for long enough to give an average.