Per Bill and Jim Galloway over at Loxahatchee Herp Hatchery, who also run Loxahatchee Rodents and Perfect Prey, and know a thing or two about ball pythons and frozen feeders:
Unless you're being ridiculous about leaving them sit out for long periods of time, room temperature thawing is a perfectly safe way to go. It's by no means the fastest method time-wise, but I can be feeding very shortly after work after the rats have thawed themselves, rather than waiting for the hot water method. Both work equally well, though, so it's just a matter of personal preference on which will be most convenient for you.What is the best way to thaw out rodents for feeding?
I prefer to let the rodents thaw to room temperature either overnight if I plan on feeding in the morning, or during the day if Im going to feed at night.
As a side note, normal rat body temperatures are between 35.9 C (96.6 F) and 37.5 C (99.5 F) (source: http://web.jhu.edu/animalcare/procedures/rat.html). Make sure when heating, you get up to at least this range since they won't recognize a room temp rat as food.











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