I have left them in the incubator for several days after leaving eggs. As far as yolk goes if they have a yolk sac still attached then you can leave them in incubator untill they absorb it or put them on a damp paper towel in hatchling tub. Most that leave the egg on there own have already aborbed the sac. When you check the bellies you will either see a slit where sac was or the sac still attached.