Surprisingly, I have never seen any information about when the best time of day is to handle any species of snake for best temperament (and other factors). So, I thought this would be an interesting learning experience. Personally, I handle all of my snakes at all different times of the day. Though, most of the time it ends up being between the hours of 5:00 PM and 11:00 PM. I can't say if I have ever noticed any difference in any of their temperaments, whether it is in the morning, afternoon, evening or night time. With Bloods and Short Tails being nocturnal, logic tells me that evening or night time would probably be best, I guess? Perhaps there would be more of a chance of startling or stressing them if they are awakened from their ssslumber?