Ball Python is suddenly aggressive

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Originally Posted by BCS

Feeding in a separate enclosure is a false myth BTW. People say that it keeps them from getting aggressive while in their enclosure. I have actually noticed people have more issues when feeding in a separate enclosure.

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This. You've actually taught her over time that your hand entering her enclosure means that she's likely to be fed soon.

I would also suggest weighing her and also weighing her feeder. Depending on the source there can be a pretty wide discrepancy in the size of a "medium" rat. If she's a 2000+ gram female and her medium rat is right at 85 grams, she'll be hungry again really quickly.