Genotyping and QTL project

Hi everyone,

I am new to the BP forums but I have had an idea for a while to do a BP genotyping and QTL study with various morphs. While sequencing the entire BP genome would be expensive and tedious a simple molecular marker polymorphism study with different morphs would be pretty cheap. I was wondering if any of you in here with me are molecular biologists and if we could coordinate our efforts to put out a paper in MGG or BMC Genomics.

Dr. Said.

Um...welcome I guess? All I know about genetics is the simple co-dom, dom, and reccessive trait type things.

Genetic Markers are all most of us would be interested in. However projections for the cost have never been cheap from what I have seen. Let alone someone organizing the effort that has access to equipment.

It would be a really cool project to get involved in. I've been looking for an internship ;) Hahaha

I'd like to see someone do a simple karyotyping study on banana/coral glow ball pythons to see if they have the normal two sex chromosomes or three, as has sometimes been suggested.

I have a PhD in biochemistry and my lab had a massive genetic mapping project when I was doing my PhD. I don't think it would be as simple as you believe. As far as I know there isn't much if any mapping of the BP genome.

I've thought about this and I don't really see a point to it, other than intellectual curiosity (I'd love to know the BEL complex pathway and how so many mutations affect a single gene with massively different outcomes). But beyond that, so what? As someone who thinks we spend much much too little money on basic research and should fund a heck of a lot more, this isn't something I would consider a valuable use of resources. Just my :2cent:.

I have no info on how difficult or expensive it would be although I do get really get excited by the mass media reports of increasing technology, speed and decreasing cost in the genetic field. Sure it probably will not cure cancer or solve world hunger but I also am vary curious about the genetics behind the white snake alleles and the coral glow/banana sex bias. Seems inevitable that if the resources keep getting more and more available someone will eventually take this up just because it interests them.

you could use the burmese python genome to scaffold the BP genome....so it wouldn't be a complete de novo assembly. I'm not sure how bad it would be in terms of cost to assemble the genome. Do you have funding for this?

I have a lot of experience with genomics and bioinformatics but i've not yet tried to assemble a genome...

I'm not sure how deep you would need to sequence to do this. 100 bp single end hiseq is down to about a thousand bucks a lane.

I'm open minded about this.