Earlier today I put Frontline spray on all three rats, dabbing it on their backs, bellies and legs with a q-tip. Tonight I went in to see them and I can't find any mites! I could not find a single mite on all three rats.

So what should be the next step? I'm going to clean out their cage tomorrow really well. Should I spray the new bedding down a bit just in case?

I am really starting to love these little guys. Whenever I open the cage, Nibble and Monk come running up, putting their little paws on my fingers and Monk tends to crawl up into my hand. Nibble I have to pick up but both love to come out and crawl around. Still working on Kasper. Any suggestions on how to get her to be less frightened?